Western Blot Substrates and Substrate Kits
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Revvity Health Sciences Inc Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Seracare Life Sciences Inc PHOSPHAGLORESERVE AP SUBSTRATE
PhosphaGLO Reserve AP Substrate is the chemiluminescent substrate of choice when greatest senstivity is required for detection of alkaline-phosphatase (AP) in Western blotting or ELISA. Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Other features and benefits include: Excellent sensitivity; detect femotogram levels of protein. Long glow times up to five days; allows for repeated exposures (Western Blot) or luminosity readings (ELISA). Easy-to-follow protocol; simply add to microwell or blot and detect within minutes. Product is stable for two years at 4deg. C; no worries about rapidly degrading substrates. Compatible with film or chemiluminescent imager; provides flexibility for recording permanent results.
Seracare Life Sciences Inc PHOSPHAGLO AP CHEMI SUBSTRATE
PhosphaGLO AP Substrate is the chemiluminescent substrate of choice for easy, reliable detection of picogram protein levels via alkaline-phosphatase (AP) in Western blotting or ELISA. Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Other features and benefits include: Long glow times up to five days; allows for repeated exposures (Western Blot) or luminosity readings (ELISA). Easy-to-follow protocol; simply add to microwell or blot and detect within minutes. Easily interchangable with other AP chemiluminescent substrates; requires little optimization to switch. Product is stable for two years at 4deg. C; no worries about rapidly degrading substrates. Compatible with film or with a chemiluminescent imager; provides flexibility for recording permanent results. Cost effective; PhosphaGLO offers cost savings per blot when compared to other chemiluminescent AP substrates.
Biotium DNA Binding Dyes DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 1/EA
The EMBER Ultra DNA Gel Kit is optimized for superior DNA sensitivity and resolution using ultra-sensitive EMBER dye The kit includes EMBER Ultra Precoated Agarose and EMBER Ultra DNA Loading Dye Ideal for blue LED imagers also compatible with UV gel boxes Trial size kit makes approximately 10 minigels
FUJIFILM BIOSCIENCES INC SCALEVIEW S Trial Kit
SCALEVIEW-S Trial Kit consists of tissue clearing reagents used for ScaleS a tissue clearing method developed by Dr Atsushi Miyawaki and his colleagues This method uses solutions with mainly urea The tissue is made transparent and adjusted RI for microscopic observation by soaking in 6 kinds of solutions SCALEVIEW-S0 SCALEVIEW-S1 SCALEVIEW-S2 SCALEVIEW-S3 SCALEVIEW-S4 SCALEVIEW-SMt 2mm section can be made transparent in 1 day using the reagents The method can make a transparent tissue keeping the structure of proteins Therefore it is possible to observe the tissue expressing fluorescent proteins and to label by antibody and/or fluorescent dye after tissue clearing Its also applicable to not only mouse and rat brain but also human after dye The transparent tissue can be observed for electric microscope or confocal microscope
Genetex Inc Trident pico Western HRP Substrate
Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.